phenotypic and genotypic detection of metallo-beta-lactamases among imipenem resistant gram negative isolates
نویسندگان
چکیده
background : imipenem-resistant gram negative bacteria, resulting from metallo-beta-lactamase (mbls)-producing strains have been reported to be among the important causes of nosocomial infections and of serious therapeutic problem worldwide. because of their broad range, potent carbapenemase activity and resistance to inhibitors, these enzymes can confer resistance to almost all beta-lactams. the prevalence of metallo-beta-lactamase among imipenem-resistant acinetobacter spp., pseudomonas spp. and enerobacteriaceae isolates is determined. methods : in this descriptive study 864 clinical isolates of acinetobacter spp., pseudomonas spp. and enterobacteriaceae , were initially tested for imipenem susceptibility. the metallo-beta-lactamase production was detected using combined disk diffusion, double disk synergy test, and hodge test. then all imipenem resistant isolates were tested by pcr for imp , vim and ndm genes. results : among 864 isolates, 62 (7.17 %) were imipenem-resistant. positive phonetypic test for metallo-beta-lactamase was 40 (64.5%), of which 24 (17.1%) and 16 (9.2%) isolates were acinetobacter spp. and pseudomonas spp., respectively. by pcr method 30 (48.4%) of imipenem resistant acinetobacter, and pseudomonas isolates were positive for mbl-producing genes. none of the enterobacteriaceae isolates were positive for metallo-beta-lactamase activity. conclusion : the results of this study are indicative of the growing number of nosocomial infections associated with multidrug-resistant gram negative bacteria in this region leading to difficulties in antibiotic therapy. thereby, using of phenotypic methods can be helpful for management of this problem.
منابع مشابه
phenotypic and genotypic detection of metallo-beta-lactamases among imipenem-resistant gram negative isolates
background : imipenem-resistant gram negative bacteria, resulting from metallo-beta-lactamase (mbls)-producing strains have been reported to be among the important causes of nosocomial infections and of serious therapeutic problem worldwide. because of their broad range, potent carbapenemase activity and resistance to inhibitors, these enzymes can confer resistance to almost all beta-lactams....
متن کاملPhenotypic and Genotypic Detection of Metallo-Beta-Lactamases in Carbapenem Resistant Acinetobacter baumannii
متن کامل
Detection of Metallo-Beta Lactamases Among Carbapenem-Resistant Pseudomonas aeruginosa
BACKGROUND Carbapenems are important drugs used for the treatment of Pseudomonas aeruginosa infections, however metallo-β-lactamases (MBL) are able to efficiently hydrolyze these classes of drugs. Immediate detection of the MBL-producing P. aeruginosa is necessary in order to accurately treat infections caused by this organism. OBJECTIVES To determine the prevalence of MBL producing P. aerugi...
متن کاملUsefulness of phenotypic and genotypic methods for metallo-beta-lactamases detection in carbapenem-resistant Acinetobacter baumannii strains
BACKGROUND Acinetobacter baumannii is an opportunistic microorganism with an increasing role in nosocomial outbreaks. For the last 2 decades, a growing number of carbapenem-resistant A. baumannii strains have been identified, including the metallo-beta-lactamases (MBLs) producers. The study aimed to investigate the genetic relatedness of, and MBLs production among, a collection of A. baumannii ...
متن کاملdetection of metallo-beta lactamases among carbapenem-resistant pseudomonas aeruginosa
conclusions epidemiological and regional evaluation of mbl-producing p. aeruginosa through simple and inexpensive methods should be considered for effective treatment of carbapenem-resistant p. aeruginosa infections. results amongst all the p. aeruginosa isolates, 58.7% were resistant to imipenem while 31.8%, 13.5% and 74.4% were resistant to meropenem, doripenem and ertapenem, respectively. am...
متن کاملMetallo‐beta‐lactamases among imipenem‐resistant Pseudomonas aeruginosa in a brazilian university hospital
INTRODUCTION Imipenem-resistant Pseudomonas aeruginosa resulting from metallo-β-lactamases has been reported to be an important cause of nosocomial infection and is a critical therapeutic problem worldwide, especially in the case of bacteremia. OBJECTIVES To determine the frequency of metallo-β-lactamases among imipenem-resistant Pseudomonas aeruginosa isolates and to compare methods of pheno...
متن کاملمنابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
journal of medical bacteriologyجلد ۵، شماره ۱-۲، صفحات ۳۶-۴۲
کلمات کلیدی
میزبانی شده توسط پلتفرم ابری doprax.com
copyright © 2015-2023